Antioxidant Activity of Rosemary Extract in Comparison with Synthetic Antioxidant on the Stability of Refined Sesame Oil During Accelerated Storage Time

Document Type : Original research

Authors

1 Department of Health & Food Quality Control, Faculty of Veterinary Medicine, Ardakan University, P.O. Box 184, Ardakan, Iran.

2 Department of Health & Food Quality Control, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.

3 Bioprocessing and Biodetection Lab, Department of Food Science and Technology, University of Tehran, Karaj, Iran

4 Department of Food Science, Islamic Azad University, Yazd, Iran.

10.22059/jfabe.2026.403452.1216

Abstract

Rosemary extract is widely recognized as a natural antioxidant, exhibiting strong antioxidant activity due to its components. In this study antioxidant efficacy of different concentrations of Rosemary Officinalis extract against lipid oxidation of refined sesame oil was evaluated during 30 day storage at 60 ⁰C. The leaves of R. Officinalis and refined sesame oil were obtained from Yazd province and extracted with ethanol. The antioxidant activity of ethanolic rosemary extract (RE) was evaluated using DPPH radical scavenging capacity, Rancemit analysis, Peroxide value (PV) measurement and Thiobarbituric acid assay (TBA). BHA as a synthetic antioxidant, was used as a positive control group. In the present study, radical-scavenging capabilities (DPPH) of RE were significantly higher than the group without RE but it was observed to be less than that of BHA. The induction period (IP) of sesame oil inoculated with rosemary extract was significantly higher than the control group and oils with synthetic antioxidants. All concentrations of Rosemary ethanol extract were able to reduce the peroxide value, TBA, especially in the long-time storage procedure.Moreover, the optimum concentrations of rosemary ethanol extract in refined sesame oil during accelerated storage were 50 and 100 μg/ml and the synthetic antioxidant expressed significantly more antioxidant activity than RE (P≤0.05). Results of the present study suggest that ethanol rosemary extract can act as an impressive and effective alternative to synthetic antioxidants.

Keywords

Main Subjects


Articles in Press, Accepted Manuscript
Available Online from 14 February 2026