Document Type : Original research


Department of food Science and Technology, Ramin Agriculture and Natural Resources University, Mollasani, Ahvaz, Iran


In this work the chemical composition and antimicrobial activity of Pelargonium roseum leaves essential oil that grown in southwest of Iran were determined. The hydrodistilled volatile components were identified by GC/MS analysis. A total of 33 components have been identified in the oil, representing 99.38% of the total oil and citronellol (44.63%), citronellyl formate (14.22%), isomenthon (6.34%), geraniol (5.30%) and caryophyllene (3.55%) were the main components, respectively. Antimicrobial property of essential oil was investigated by disc diffusion method against three Gram positive (Staphylococcus aureus, Bacillus ceruse, Streptococcus faecium) and four Gram negative (Escherichia coli, Salmonella typhi, Shigella dysenteria, Pseudomonas aeruginosa) bacteria and a yeast (Sacharomyces cervisiae) in comparable with the standard antibiotics. The essential oil of P. roseum showed inhibitory activity against all the bacteria and yeast tested to varying degree. B. cereus and S. faecium were found as the most sensitive and resistant bacteria, respectively. According to these results, it can be concluded that the essential oil of P. roseum has suitable spectrum antimicrobial activity and can be used as natural preservatives in food industry.


Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils–a review. Food and chemical toxicology46(2), 446-475.
Brul, S., & Coote, P. (1999). Preservative agents in foods: mode of action and microbial resistance mechanisms. International journal of food microbiology50(1-2), 1-17.
Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International journal of food microbiology94(3), 223-253.
Celiktas, O. Y., Kocabas, E. H., Bedir, E., Sukan, F. V., Ozek, T., & Baser, K. H. C. (2007). Antimicrobial activities of methanol extracts and essential oils of Rosmarinus officinalis, depending on location and seasonal variations. Food Chemistry100(2), 553-559.
Cosentino, S. C. I. G., Tuberoso, C. I. G., Pisano, B., Satta, M. L., Mascia, V., Arzedi, E., & Palmas, F. (1999). In‐vitro antimicrobial activity and chemical composition of Sardinian thymus essential oils. Letters in applied microbiology29(2), 130-135.
Dabiri, M., Sefidkon, F., Yousefi, M., & Bashiribod, S. (2011). Volatile components of Pelargonium roseum R. Br. Journal of Essential Oil Bearing Plants14(1), 114-117.
Del Rosario, C. S., Tulin, E. E., & Estremos, D. V. (1992). Screening of microorganisms for terpene degradation potential. Philippine Journal of Crop Science17(2), 85-88.
Elmann, A., Mordechay, S., Rindner, M., & Ravid, U. (2010). Anti-neuroinflammatory effects of geranium oil in microglial cells. Journal of Functional Foods2(1), 17-22.
Ghannadi, A., Bagherinejad, M. R., Abedi, D., Jalali, M., Absalan, B., & Sadeghi, N. (2012). Antibacterial activity and composition of essential oils from Pelargonium graveolens L'Her and Vitex agnus-castus L. Iranian journal of microbiology4(4), 171.
Gomes, P. B., Mata, V. G., & Rodrigues, A. E. (2004). Characterization of Portuguese-grown geranium oil (Pelargonium sp.). Journal of Essential Oil Research16(5), 490-495.
Knobloch, K., Pauli, A., Iberl, B., Weigand, H., & Weis, N. (1989). Antibacterial and antifungal properties of essential oil components. Journal of Essential Oil Research1(3), 119-128.
Kotan, R., Kordali, S., & Cakir, A. (2007). Screening of antibacterial activities of twenty-one oxygenated monoterpenes. Zeitschrift für Naturforschung C62(7-8), 507-513.
Owlia, P., Saderi, H., Rasooli, I., & Sefidkon, F. (2010). Antimicrobial characteristics of some herbal Oils on Pseudomonas aeruginosa with special reference to their chemical compositions. Iranian Journal of Pharmaceutical Research, 107-114.
Rota, M. C., Herrera, A., Martínez, R. M., Sotomayor, J. A., & Jordán, M. J. (2008). Antimicrobial activity and chemical composition of Thymus vulgaris, Thymus zygis and Thymus hyemalis essential oils. Food control19(7), 681-687.
Rusenova N & Parvanov P (2009) Antimicrobial activities of twelve essential oils against microorganisms of veterinary importance. Trakia J Sci. 7(1), 37-43.
Sikkema, J., Weber, F. J., Heipieper, H. J., & Bont, J. A. D. (1994). Cellular toxicity of lipophilic compounds: mechanisms, implications, and adaptations. Biocatalysis10(1-4), 113-122.
Skocibusic, M., Bezic, N., & Dunkic, V. (2006). Phytochemical composition and antimicrobial activities of the essential oils from Satureja subspicata Vis. growing in Croatia. Food Chem96(1), 20-28.
Southwell, I. A., & Stiff, I. A. (1995). Chemical composition of an Australian geranium oil. Journal of Essential Oil Research7(5), 545-547.
Tozoni, D., Zacaria, J., Vanderlinde, R., Delamare, A. P. L., & Echeverrigaray, S. (2010). Degradation of citronellol, citronellal and citronellyl acetate by Pseudomonas mendocina IBPse 105. Electronic Journal of Biotechnology13(2), 2-3.
Verma, R. S., Verma, R. K., Yadav, A. K., & Chauhan, A. (2010). Changes in the essential oil composition of rose-scented geranium (Pelargonium graveolens L’Herit. ex Ait.) due to date of transplanting under hill conditions of Uttarakhand.
World Health Organization, & World Health Organization. Reproductive Health. (2010). Medical eligibility criteria for contraceptive use. World Health Organization.
Zandi-Sohani, N., Hojjati, M., & Carbonell-Barrachina, A. A. (2012). Volatile composition of the essential oil of Callistemon Citrinus leaves from Iran. Journal of Essential oil Bearing Plants15(5), 703-707.