Document Type : Original research


1 Nutrition and Food Sciences Research Center, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

2 Iranian Space Agency, Iranian Space Research Center, Institute of Mechanics, Shiraz, Iran


Sugarcane bagasse contains cellulose, lignin and hemicellulose, 39-42%, 20-25% and 25-27% respectively. So it is can be used as a sugar source in many processes. Lignin and hemicellulose must be removed before hydrolysis of cellulose. Several different pretreatment approaches have been studied. The purpose of this research is comparison of acid, ozone and combination of ozone-acid as pretreatment methods for improving enzymatic digestibility of sugar cane bagasse in pilot plant scale. Sugar cane Bagasse was washed and pretreated by sulfuric acid (BA) 0.1 %, sodium hydroxide (BSH) 0.1% and (BS) steam was done at pressure 2 bar. Sugar cane bagasse was also treated by sulfuric acid (1, 2, 2.5, and 5%) at 121 ºC and 151 ºC. Ozone and ozone-acid methods also was used for delignification of bagasse. Particle diameter of bagasse in all pretreatment methods was 3-4 mm. All pretreated bagasse was hydrolyzed by cellulose enzyme complex and beta-gluocosidase at pH 5 and temperature 45 ºC. Glucose and xylose content of hydrolyzed sample was analyzed by high performance liquid chromatography (HPLC). Acid is the best media for pretreatment of bagasse in comparison with steam and base. The data showed that high concentration of acid had indirect effect on yield of sugar production in hydrolysis step. Furthermore ozonolysis pretreatment of bagasse led to higher amount of glucose in comparison with acid and acid-ozone methods. Moisture content and duration of ozonation had significant effect on sugar content of hydrolyzed solution and sugar content of hydrolyzed samples was 6%.


Badger, P. C. (2002). Ethanol from cellulose: a general review. Trends in New Crops and New Uses, 1, 17–21.
Gámez, S., González-Cabriales, J. J., Ramírez, J. A., Garrote, G., & Vázquez, M. (2006). Study of the hydrolysis of sugar cane bagasse using phosphoric acid. Journal of Food Engineering, 74(1), 78–88.
García-Cubero, M. T., González-Benito, G., Indacoechea, I., Coca, M., & Bolado, S. (2009). Effect of ozonolysis pretreatment on enzymatic digestibility of wheat and rye straw. Bioresource Technology, 100(4), 1608–1613.
Hahn-HäGerdal, B., Lindén, T., Senac, T., & Skoog, K. (1991). Ethanolic fermentation of pentoses in lignocellulose hydrolysates. Applied Biochemistry and Biotechnology, 28(1), 131–144.
Hamelinck, C. N., Van Hooijdonk, G., & Faaij, A. P. C. (2005). Ethanol from lignocellulosic biomass: techno-economic performance in short-, middle-and long-term. Biomass and Bioenergy, 28(4), 384–410.
Hu, Z., & Wen, Z. (2008). Enhancing enzymatic digestibility of switchgrass by microwave-assisted alkali pretreatment. Biochemical Engineering Journal, 38(3), 369–378.
Imai, M., Ikari, K., & Suzuki, I. (2004). High-performance hydrolysis of cellulose using mixed cellulase species and ultrasonication pretreatment. Biochemical Engineering Journal, 17(2), 79–83.
Jeffries, T. W. (2006). Engineering yeasts for xylose metabolism. Current Opinion in Biotechnology, 17(3), 320–326.
Keshwani, D. R., & Cheng, J. J. (2010). Microwave‐based alkali pretreatment of switchgrass and coastal bermudagrass for bioethanol production. Biotechnology Progress, 26(3), 644–652.
Quesada, J., Rubio, M., & Gómez, D. (1999). Ozonation of lignin rich solid fractions from corn stalks. Journal of Wood Chemistry and Technology, 19(1–2), 115–137.
Sun, Y. E., & Cheng, J. J. (2005). Dilute acid pretreatment of rye straw and bermudagrass for ethanol production. Bioresource Technology, 96(14), 1599–1606.
Sun, Y., & Cheng, J. (2002). Hydrolysis of lignocellulosic materials for ethanol production: a review. Bioresource Technology, 83(1), 1–11.
Vidal, P. F., & Molinier, J. (1988). Ozonolysis of lignin-improvement of in vitro digestibility of poplar sawdust. Biomass, 16(1), 1–17.
Weil, J., Westgate, P., Kohlmann, K., & Ladisch, M. R. (1994). Cellulose pretreaments of lignocellulosic substrates. Enzyme and Microbial Technology, 16(11), 1002–1004.