Document Type : Original research

Authors

• Helma Dashlaali ¹
• maryam moslehishad ²
• Fatemeh Ghamari ³
• mohammad Mohammadi ⁴

¹ Department of Food Science and Technology, Medical Branch, Islamic Azad University, Tehran, Ira

² Department of Food Science & Technology, Safadasht Branch, Islamic Azad University, Tehran, Iran

³ Department of Science Payame Noor University, P.O.Box 19395-4697,Iran

⁴ Assistant professor Department of science payame noor university ,P.O.Box 19395-4697,Tehran,Iran

Abstract

ABSTRACT

This research aimed to evaluate the antimicrobial, antioxidant, and lipase inhibitory properties of leaf extracts from Moringa Pergerinaperegrina (M. peregrina) and Murraya koenigii (M. koenigii). Antioxidant capacity was assessed using the ABTS method, while total phenol content was measured via the Folin-Ciocalteu method. The antifungal activity against Candida albicans was determined through zone inhibition assays, and the ability to inhibit Helicobacter pylori was evaluated by measuring urease enzyme activity. Lipase activity inhibition was assessed using ELISA at 405 nm. Analysis of variance revealed that increasing concentrations of both extracts significantly enhanced antioxidant activity and total phenol content (p<0.05). MoringaM. peregrinaPergerina and M. koenigiiMurraya koenigii exhibited the highest antioxidant activity at 4000 mg/L, while M. koenigiiMurraya koenigii showed the lowest activity at 31.25 mg/L. The highest total phenol content occurred at 4000 ppmmg/L for M. koenigiiMurraya koenigii. Synergistic effects of both extracts were noted at 1000 ppmmg/L. At 1500 ppmmg/L, MoringaM. peregrinaPergerina displayed the largest inhibition zone against Candida albicans; conversely, M. koenigiiMurraya koenigii showed the strongest inhibition of Helicobacter pylori at 2000 ppmmg/L. The greatest lipase inhibitory activity was found at 4000 ppmmg/L for MoringaM. peregrinapergerina, while M. koenigiiMurraya koenigii had the least at 2000 ppmmg/L, and there were no synergistic effects. These findings suggest that MoringaM. peregrinapergerina and M. koenigiiMurraya koenigii leaf extracts have potential applications in developing anti-diabetic food products.

Keywords

• Antibacterial Activity
• Antioxidant
• Lipase Inhibitory
• Murraya koenigii
• Moringa peregrina

Main Subjects

• Functional fermented foods or ingredients