Journal of Food and Bioprocess Engineering

Comparing total anthocyanins, total phenolics and antioxidant activities of extracts (aqueous, organic and anthocyanin) obtained from pomegranate (peel, juice, and seed) and antimicrobial activity of peel extracts on the four pathogenic bacteria

Document Type : Original research

Authors

1 Department of Food Science and Engineering, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

2 Innovative Technologies in Functional Food Production Research Center, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

Abstract

Nowadays, consumers are highly worried about using chemical preservatives in foods. Thus they tend to use natural and safe food products with healthful benefits. Pomegranate and its peel can have such a role. This study aimed to determine the antioxidant activity, total phenolics, and flavonoids properties of different parts of pomegranate extracts. Three types of extracts were prepared with different solvent (water extract, organic and anthocyanin extract). The total phenolic and antioxidant activities were highest in peels, intermediate in juice and lowest in seeds and total anthocyanin was highest in juices. The organic extracts have the highest antioxidant activity. Then we examined the antimicrobial activity of peel extracts (organic, aqueous and anthocyanin extracts) and determined the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) on two gram-positive bacteria Staphylococcus aureus and Bacillus cereus and two gram-negative bacteria E. coli and Salmonella typhi using liquid dilution susceptibility testing method. Thus, 62.5 ppm MIC of the organic and anthocyanin extracts of the peel was useful in Staphylococcus aureus and Salmonella typhi bacteria. Furthermore, organic and anthocyanin extracts of the peel at a concentration of 125 ppm had bactericidal effects on Staphylococcus aureus and E. coli bacteria and Bacillus cereus and Salmonella typhi at a concentration of 250 ppm. Thus, one can state that pomegranate peel extracts have high antibacterial effects because of high phenolic compounds and high levels of anthocyanin with high antioxidant activities.

Keywords

Abid, M., Yaich, H., Cheikhrouhou, S., Khemakhem, I., Bouaziz, M., Attia, H., & Ayadi, M. A. (2017). Antioxidant properties and phenolic profile characterization by LC–MS/MS of selected Tunisian pomegranate peels. Journal of food science and technology, 54(9), 2890-2901.
Algurairy, A. T. M. (2018). Assessing the antibacterial activity of pomegranate against Staphylococcus aureus obtained from wound infections. Research journal of pharmaceutical biological and chemical sciences, 9(4), 1602-1606.
Baron, J. O., & Finegold, M. (1990). Diagnostic microbiology 8th edition. The CV Company, New York, 435-433.
Çam, M., Hışıl, Y., & Durmaz, G. (2009). Classification of eight pomegranate juices based on antioxidant capacity measured by four methods. Food chemistry, 112(3), 721-726.
Chaudhar, A., & Rahul, S. N (2017). Antibacterial activity of Punica granatum (Pomegranate) fruit peel extract against pathogenic and drug resistance bacterial strains.  International journal of current microbiology applied science, 6(12), 3802-3807.
Conner, H. T., Dumas, D. H., & Nelson, L. L. (1993). U.S. Patent No. 5,176,794. Washington, DC: U.S. Patent and Trademark Office.
Côté, J., Caillet, S., Dussault, D., Sylvain, J. F., & Lacroix, M. (2011). Effect of juice processing on cranberry antibacterial properties. Food research international, 44(9), 2922-2929.
Dahham, S. S., Ali, M. N., Tabassum, H., & Khan, M. (2010). Studies on antibacterial and antifungal activity of pomegranate (Punica granatum L.). American-Eurasian Journal of Agricultural & Environmental Sciences, 9(3), 273-281.
Ferrazzano, G. F., Scioscia, E., Sateriale, D., Pastore, G., Colicchio, R., Pagliuca, C., & Scaglione, E. (2017). In vitro antibacterial activity of pomegranate juice and peel extracts on cariogenic bacteria. BioMed research international, 2017.
Ghasemian, A., Mehrabian, S., & Majd, A. (2006). Peel extracts of two Iranian cultivars of pomegranate (Punica granatum) have antioxidant and antimutagenic activities. Pakistan journal of biological science, 7, 1402-405.
Ghasemzadeh, A., Jaafar, H. Z., & Rahmat, A. (2010). Antioxidant activities, total phenolics and flavonoids content in two varieties of Malaysia young ginger (Zingiber officinale Roscoe). Molecules, 15(6), 4324-4333.
Hmid, I., Elothmani, D., Hanine, H., Oukabli, A., & Mehinagic, E. (2017). Comparative study of phenolic compounds and their antioxidant attributes of eighteen pomegranate (Punica granatum L.) cultivars grown in Morocco. Arabian Journal of Chemistry, 10, S2675-S2684.
Holley, R. A., & Patel, D. (2005). Improvement in shelf-life and safety of perishable foods by plant essential oils and smoke antimicrobials. Food microbiology, 22(4), 273-292.
Kaur, G., Jabbar, Z., Athar, M., & Alam, M. S. (2006). Punica granatum (pomegranate) flower extract possesses potent antioxidant activity and abrogates Fe-NTA induced hepatotoxicity in mice. Food and chemical toxicology, 44(7), 984-993.
Lako, J., Trenerry, V. C., Wahlqvist, M., Wattanapenpaiboon, N., Sotheeswaran, S., & Premier, R. (2007). Phytochemical flavonols, carotenoids and the antioxidant properties of a wide selection of Fijian fruit, vegetables and other readily available foods. Food Chemistry, 101(4), 1727-1741.
Lansky, E. P., & Newman, R. A. (2007). Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of ethnopharmacology, 109(2), 177-206.
Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., & Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food chemistry, 96(2), 254-260.
Lucas, D. L., & Were, L. M. (2009). Anti–Listeria monocytogenes activity of heat-treated lyophilized pomegranate juice in media and in ground top round beef. Journal of food protection, 72(12), 2508-2516.
Mousavinejad, G., Emam-Djomeh, Z., Rezaei, K., & Khodaparast, M. H. H. (2009). Identification and quantification of phenolic compounds and their effects on antioxidant activity in pomegranate juices of eight Iranian cultivars. Food Chemistry, 115(4), 1274-1278.
Naz, S., Siddiqi, R., Ahmad, S., Rasool, S. A., & Sayeed, S. A. (2007). Antibacterial activity directed isolation of compounds from Punica granatum. Journal of food science, 72(9), M341-M345.
Negi, P. S., & Jayaprakasha, G. K. (2003). Antioxidant and antibacterial activities of Punica granatum peel extracts. Journal of food science, 68(4), 1473-1477.
Neto, C. C., Krueger, C. G., Lamoureaux, T. L., Kondo, M., Vaisberg, A. J., Hurta, R. A., & Reed, J. D. (2006). MALDI‐TOF MS characterization of proanthocyanidins from cranberry fruit (Vaccinium macrocarpon) that inhibit tumor cell growth and matrix metalloproteinase expression in vitro. Journal of the science of food and agriculture, 86(1), 18-25.
Okonogi, S., Duangrat, C., Anuchpreeda, S., Tachakittirungrod, S., & Chowwanapoonpohn, S. (2007). Comparison of antioxidant capacities and cytotoxicities of certain fruit peels. Food chemistry, 103(3), 839-846.
Olsen, S. J., MacKinon, L. C., Goulding, J. S., Bean, N. H., & Slutsker, L. (2000). Surveillance for foodborne-disease outbreaks, United States, 1993-1997.
Osman, H., Rahim, A., Isa, N., & Bakhir, N. (2009). Antioxidant activity and phenolic content of Paederia foetida and Syzygium aqueum. Molecules, 14(3), 970-978.
Oussalah, M., Caillet, S., Saucier, L., & Lacroix, M. (2007). Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157: H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food control, 18(5), 414-420.
Praven, K., Ramamoorty, A., & Awang, B. (2007). Anti-oxidant activity, total phenolic and flavonoid content Morinda citrifolia fruit. Journal of engineering science, 2, 70-80.
Rosas‐Burgos, E. C., Burgos‐Hernández, A., Noguera‐Artiaga, L., Kačániová, M., Hernández‐García, F., Cárdenas‐López, J. L., & Carbonell‐Barrachina, Á. A. (2017). Antimicrobial activity of pomegranate peel extracts as affected by cultivar. Journal of the science of food and agriculture, 97(3), 802-810.
Sadeghian, A., Ghorbani, A., Mohamadi-Nejad, A., & Rakhshandeh, H. (2011). Antimicrobial activity of aqueous and methanolic extracts of pomegranate fruit skin. Avicenna Journal of Phytomedicine, 1(2), 67-73.
Schubert, S. Y., Lansky, E. P., & Neeman, I. (1999). Antioxidant and eicosanoid enzyme inhibition properties of pomegranate seed oil and fermented juice flavonoids. Journal of ethnopharmacology, 66(1), 11-17.
Seeram, N. P., Adams, L. S., Hardy, M. L., & Heber, D. (2004). Total cranberry extract versus its phytochemical constituents: antiproliferative and synergistic effects against human tumor cell lines. Journal of agricultural and food chemistry, 52(9), 2512-2517.
Singh, R. P., Chidambara Murthy, K. N., & Jayaprakasha, G. K. (2002). Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models. Journal of agricultural and food chemistry, 50(1), 81-86.
Todaro, A., Cavallaro, R., La Malfa, S., Continella, A., Gentile, A., Fischer, U., & Spagna, G. (2016). Anthocyanin profile and antioxidant activity of freshly squeezed pomegranate (Punica granatum L.) juices of sicilian and spanish provenances. Italian journal of food science, 28(3), 464-479.
Viuda‐Martos, M., Fernández‐López, J., & Pérez‐Álvarez, J. A. (2010). Pomegranate and its many functional components as related to human health: a review. Comprehensive reviews in food science and food safety, 9(6), 635-654.
Wu, V. C. H., Qiu, X., Bushway, A., & Harper, L. (2008). Antibacterial effects of American cranberry (Vaccinium macrocarpon) concentrate on foodborne pathogens. LWT-Food Science and Technology, 41(10), 1834-1841.
Journal of Food and Bioprocess Engineering
Volume 2, Issue 1
June 2019
Pages 7-12