Document Type : Original research


1 Department of Health, Science and Research Branch, AJA University of Medical Sciences, Tehran, Iran

2 Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran


In the present study, the fresh egg white was employed as a carrier for enhancing the aqueous solubility and antioxidant activity of the curcumin as a bioactive hydrophobic ingredient. The curcumin-egg white protein complexes were prepared at pH values of 7.0 and 3.0. The results indicated that the binding of curcumin to egg white proteins at pH values of 7.0 and 3.0 drastically improved its water solubility. The fluorescence measurements showed that the hydrophobic interactions were generated between the curcumin and proteins. The curcumin-loaded egg white prepared at pH 7.0 and 3.0 also had a good antioxidant activity that was measured by radical (ABTS and DPPH) scavenging activity and reducing power test.  In general, the findings of this study suggested that the egg white can be considered as an efficient system for increasing the aqueous solubility and antioxidant activity of curcumin which enhances its applications in different fields including food, cosmetic, and pharmaceutical industries.


Abaee, A., Mohammadian, M., & Jafari, S. M. (2017). Whey and soy protein-based hydrogels and nano-hydrogels as bioactive delivery systems. Trends in Food Science & Technology, 70, 69–81.
Abbasi Rad, S., & Askari, G. (2018). Optimization of edible Alyssum homalocarpum films for physical and mechanical properties. Journal of Food and Bioprocess Engineering, 2(1), 15–24.
Alavi, F., Emam-Djomeh, Z., Yarmand, M. S., Salami, M., Momen, S., & Moosavi-Movahedi, A. A. (2018). Cold gelation of curcumin loaded whey protein aggregates mixed with k-carrageenan: Impact of gel microstructure on the gastrointestinal fate of curcumin. Food Hydrocolloids, 85, 267–280.
Babaei, J., Khodaiyan, F., & Mohammadian, M. (2019). Effects of enriching with gellan gum on the structural, functional, and degradation properties of egg white heat-induced hydrogels. International Journal of Biological Macromolecules, 128, 94–100.
Chang, C., Meikle, T. G., Su, Y., Wang, X., Dekiwadia, C., Drummond, C. J., … Yang, Y. (2019). Encapsulation in egg white protein nanoparticles protects anti-oxidant activity of curcumin. Food Chemistry, 280, 65–72.
Chen, S., Zhang, N., & Tang, C.-H. (2016). Influence of nanocomplexation with curcumin on emulsifying properties and emulsion oxidative stability of soy protein isolate at pH 3.0 and 7.0. Food Hydrocolloids, 61, 102–112.
Li, M., Ma, Y., & Ngadi, M. O. (2013). Binding of curcumin to β-lactoglobulin and its effect on antioxidant characteristics of curcumin. Food Chemistry, 141(2), 1504–1511.
Liu, C., Yang, X., Wu, W., Long, Z., Xiao, H., Luo, F., … Lin, Q. (2018). Elaboration of curcumin-loaded rice bran albumin nanoparticles formulation with increased in vitro bioactivity and in vivo bioavailability. Food Hydrocolloids, 77, 834–842.
Liu, W., Chen, X. D., Cheng, Z., & Selomulya, C. (2016). On enhancing the solubility of curcumin by microencapsulation in whey protein isolate via spray drying. Journal of Food Engineering, 169, 189–195.
Liu, Y., Ying, D., Cai, Y., & Le, X. (2017). Improved antioxidant activity and physicochemical properties of curcumin by adding ovalbumin and its structural characterization. Food Hydrocolloids, 72, 304–311.
Mirzakhani, M., Ekrami, M., & Moini, S. (2018). Chemical composition, total phenolic content and antimicrobial activities of Zhumeria majdae. Journal of Food and Bioprocess Engineering, 2(1), 1–8.
Mohammadian, M., Salami, M., Momen, S., Alavi, F., Emam-Djomeh, Z., & Moosavi-Movahedi, A. A. (2019). Enhancing the aqueous solubility of curcumin at acidic condition through the complexation with whey protein nanofibrils. Food Hydrocolloids, 87, 902–914.
Nasabi, M., Labbafi, M., Mousavi, M. E., & Madadlou, A. (2017). Effect of salts and nonionic surfactants on thermal characteristics of egg white proteins. International Journal of Biological Macromolecules, 102, 970–976.
Pan, K., Zhong, Q., & Baek, S. J. (2013). Enhanced dispersibility and bioactivity of curcumin by encapsulation in casein nanocapsules. Journal of Agricultural and Food Chemistry, 61(25), 6036–6043.
Tapal, A., & Tiku, P. K. (2012). Complexation of curcumin with soy protein isolate and its implications on solubility and stability of curcumin. Food Chemistry, 130(4), 960–965.
Wang, L., Gulati, P., Santra, D., Rose, D., & Zhang, Y. (2018). Nanoparticles prepared by proso millet protein as novel curcumin delivery system. Food Chemistry, 240, 1039–1046.
Xiang, H., Sun-waterhouse, D., Cui, C., Wang, W., & Dong, K. (2018). Modification of soy protein isolate by glutaminase for nanocomplexation with curcumin. Food Chemistry, 268, 504–512.
Yi, J., Fan, Y., Zhang, Y., Wen, Z., Zhao, L., & Lu, Y. (2016). Glycosylated α-lactalbumin-based nanocomplex for curcumin: Physicochemical stability and DPPH-scavenging activity. Food Hydrocolloids, 61, 369–377.