Physical and mechanical features investigation of protein-based biodegradable films obtained from trout fish waste

Document Type : Original research


Transfer Phenomena Laboratory (TPL), Department of Food Science, Technology and Engineering, Faculty of Biosystems Engineering, University of Tehran


Biological packaging material based on obtained proteins from fish waste, are biopolymers that have the capability of biodegradable film formation. Thus, purpose of this research is to study and investigate some of films’ physical features made from trout fish Myofibril protein. The film forming solution containing 1.5%, 2% and 2.5% (w/v) Myofibril protein isolate of 100ml solution and glycerol as plasticizer in three levels 25%, 50%, 75% (w/w) per dry material have been used. In order to solve existing protein in solution its pH must be reach to 3 by 1molar HCl. Films have been produced by casting method for 48 hours in 25°C. Then film’s mechanical features, water vapor penetrability, films solubility in water, transparency and color were investigated. Effect of protein concentration was significantly obvious on films mechanical features, thickness, water vapor penetrability and color. Obtained results from statistical analysis data by SPSS software showed that films with 2% (w/v) dry material along with 50% (w/w) glycerol demonstrated the best mechanical features.


Andreuccetti, C., Carvalho, R. A., & Grosso, C. R. F. (2009). Effect of hydrophobic plasticizers on functional properties of gelatin-based films. Food Research International, 42(8), 1113–1121.
Chinabhark, K., Benjakul, S., & Prodpran, T. (2007). Effect of pH on the properties of protein-based film from bigeye snapper (Priacanthus tayenus) surimi. Bioresource Technology, 98(1), 221–225.
Cuq, B., Aymard, C., CUQ, J., & Guilbert, S. (1995). Edible packaging films based on fish myofibrillar proteins: formulation and functional properties. Journal of Food Science, 60(6), 1369–1374.
Cuq, B., Gontard, N., Cuq, J.-L., & Guilbert, S. (1997). Selected functional properties of fish myofibrillar protein-based films as affected by hydrophilic plasticizers. Journal of Agricultural and Food Chemistry, 45(3), 622–626.
Cuq, B., Gontard, N., CUQ, J., & Guilbert, S. (1996). Functional properties of myofibrillar protein‐based biopackaging as affected by film thickness. Journal of Food Science, 61(3), 580–584.
D882 A (Standard Test Method for Tensile Properties of Thin Plastic Philadelphia: Amercian Society for Testing and Materials) 2001.
Ekrami, M., & Emam‐Djomeh, Z. (2014). Water vapor permeability, optical and mechanical properties of salep‐based edible film. Journal of Food Processing and Preservation, 38(4), 1812–1820.
Fang, Y., Tung, M. A., Britt, I. J., Yada, S., & Dalgleish, D. G. (2002). Tensile and barrier properties of edible films made from whey proteins. Journal of Food Science, 67(1), 188–193.
Frinault, A., Gallant, D. J., Bouchet, B., & Dumont, J. P. (1997). Preparation of casein films by a modified wet spinning process. Journal of Food Science, 62(4), 744–747.
Gennadios, A. (2002). Protein-based films and coatings. CRC press.
Gontard, N., Duchez, C., CUQ, J., & GUILBERT, S. (1994). Edible composite films of wheat gluten and lipids: water vapour permeability and other physical properties. International Journal of Food Science & Technology, 29(1), 39–50.
Hagenmaier, R. D., & Shaw, P. E. (1992). Gas permeability of fruit coating waxes. Journal of the American Society for Horticultural Science, 117(1), 105–109.
Hauser, P. M., & McLaren, A. D. (1948). Permeation through and sorption of water vapor by high polymers. Industrial & Engineering Chemistry, 40(1), 112–117.
Krochta, J. M. (2002). Proteins as raw materials for films and coatings: definitions, current status, and opportunities. Protein-Based Films and Coatings, 1, 1–40.
Limpan, N., Prodpran, T., Benjakul, S., & Prasarpran, S. (2010). Properties of biodegradable blend films based on fish myofibrillar protein and polyvinyl alcohol as influenced by blend composition and pH level. Journal of Food Engineering, 100(1), 85–92.
Mu, C., Guo, J., Li, X., Lin, W., & Li, D. (2012). Preparation and properties of dialdehyde carboxymethyl cellulose crosslinked gelatin edible films. Food Hydrocolloids, 27(1), 22–29.
Nolsøe, H., & Undeland, I. (2009). The acid and alkaline solubilization process for the isolation of muscle proteins: state of the art. Food and Bioprocess Technology, 2(1), 1–27.
Osawa, R., & Walsh, T. P. (1993). Effects of acidic and alkaline treatments on tannic acid and its binding property to protein. Journal of Agricultural and Food Chemistry, 41(5), 704–707.
Paschoalick, T. M., Garcia, F. T., Sobral, P. J. do A., & Habitante, A. (2003). Characterization of some functional properties of edible films based on muscle proteins of Nile Tilapia. Food Hydrocolloids, 17(4), 419–427.
Paulo, J. do A., dos Santos, J. S., & García, F. T. (2005). Effect of protein and plasticizer concentrations in film forming solutions on physical properties of edible films based on muscle proteins of a Thai Tilapia. Journal of Food Engineering, 70(1), 93–100.
Schwartzberg, H. G. (1986). Modelling of gas and vapor transport through hydrophilic films. Food Packaging and Preservation: Theory and Practice, 115–135.
Siracusa, V., Rocculi, P., Romani, S., & Dalla Rosa, M. (2008). Biodegradable polymers for food packaging: a review. Trends in Food Science & Technology, 19(12), 634–643.
Sobral, P., Monterrey-q, E., & Habitante, A. (2002). Glass transition study of Nile Tilapia myofibrillar protein films plasticized by glycerin and water. Journal of Thermal Analysis and Calorimetry, 67(2), 499–504.
Tahergorabi, R., Beamer, S. K., Matak, K. E., & Jaczynski, J. (2012). Isoelectric solubilization/precipitation as a means to recover protein isolate from striped bass (Morone saxatilis) and its physicochemical properties in a nutraceutical seafood product. Journal of Agricultural and Food Chemistry, 60(23), 5979–5987.
Tharanathan, R. N. (2003). Biodegradable films and composite coatings: past, present and future. Trends in Food Science & Technology, 14(3), 71–78.
Zhang, Y., & Han, J. H. (2006). Plasticization of pea starch films with monosaccharides and polyols. Journal of Food Science, 71(6), E253–E261.