Anti-carcinogenic effects of Satureja khuzistanica and Zataria multiflora essential oils on K562 cell line proliferation
Document Type : Original research
Authors
1 Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran
2 Department of Food Science and Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Ahvaz, Iran
3 Department of Biochemistry and Molecular Biology, Faculty of Veterinary Sciences, Shahid Chamran University of Ahvaz, Iran
Abstract
The aim of this study was to investigate cytotoxic effect of Satureja khuzistanica and Zataria multiflora essential oils on PBMC and K562 cell lines growth inhibition. The plant essential oils were extracted by hydro-distillation and analyzed by GC-MS. In addition, the total phenol content and antioxidant activity of oils were assessed. The PBMC and K562 cell lines were cultured in RPMI medium containing 10% FBS supplemented with 0, 12.5, 25, 50 and 100 (µg/ml) of essential oils for 24, 48 and 72 h. The MTT assay revealed anti-proliferative effect of essential oils on both cell lines in a dose dependent manner. These effects became more prominent after 72 h. Both essential oils contained high amounts of phenolic compounds such as thymol and carvacrol. Due to the higher percentage of carvacrol, which has more toxicity than thymol, S. khuzistanica had a greater antioxidant activity as well as inhibitory effect on cell growth. In conclusion, the S. khuzistanica and Z. multiflora essential oils have the potential to be used in the production of anticancer medicines.
Keywords
Aizawa, K., Liu, C., Tang, S., Veeramachaneni, S., Hu, K. Q., Smith, D. E., & Wang, X. D. (2016). Tobacco carcinogen induces both lung cancer and non‐alcoholic steatohepatitis and hepatocellular carcinomas in ferrets which can be attenuated by lycopene supplementation. International journal of cancer, 139(5), 1171-1181.
Ali, M.S., Saleem, M., Ali, Z., & Ahmad, V.U. (2000). Chemistry of Zataria multiflora (Lamiaceae). Phytochemistry, 55, 933–936.
Alizadeh, A. (2017). Essential oil constituents, phenolic content and antioxidant activity of two endemic satureja species from Iran. Bangladesh Journal of Botany, 46(3), 925-931.
Alizadeh, A., & Shaabani, M. (2014). Essential oil composition, total phenolic content and antioxidant activities of Iranian Zataria multiflora Boiss. International Journal of Biosciences, 4(4), 97-104.
Amin, M., Kalantar, E., Mohammad-Saeid, N., & Ahsan, B. (2010). Antibacterial effect and physicochemical properties of essential oil of Zataria multiflora Boiss. Asian Pacific Journal of Tropical Medicine, 3(6), 439-442
Anand, P., Sundaram, C., Jhurani, S., Kunnumakkara, AB., & Aggarwal, BB. (2008). Curcumin and cancer: an “old-age” disease with an “age old” solution. Journal of Cancer Letters, 267,133-164.
Aslani, E., Naghsh, N., & Ranjbar. M. (2014). Cytotoxic effects of hydro-alcoholic extracts of cress (Lepidium Sativum) – made from different stages of the plant - on k562 Leukemia cell line. Hormozgan Medical Journal, 18(5), 370-378.
Boyum, A. (1968). Separation of leukocytes from blood and bone marrow. Introduction. Scandinavian Journal of Clinical Laboratory Investigation, 97, 7-11.
Council of Europe. (1997). European Pharmacopoeia, 3rd Edition. Strasbourg, France: Council of Europe.
Dutta, D. D., Parimala, G., Devi, S.S., & Chakraborty, T. (2011). Effect of thymol on peripheral blood mononuclear cell PBMC and acute promyelotic cancer cell line HL-60. Chemico Biological Interaction, 193, 97–106.
Ghazanfari, G., Minaie, B., Yasa, N., Nakhai, L. A., Mohammadirad, A., Nikfar, S., ... & Salehnia, A. (2006). Biochemical and histopathological evidences for beneficial effects of Satureja Khuzestanica Jamzad essential oil on the mouse model of inflammatory bowel diseases. Toxicology mechanisms and methods, 16(7), 365-372.
Gohari, A.R., Hadjiakhoondi, A., Sadat-Ebrahimi, S.E., Saeidnia, S., & Shafiee, A. (2005). Cytotoxic terpenoids from Satureja macrantha CA Mey. Daru- Journal of Faculty of Pharmacy, 13(4), 177–18.
Hassanpour, S.H. & Dehghani, M. (2017). Review of cancer from perspective of molecular. Journal of Cancer Research and Practice, 4(4), 127-129.
He, L., Mo, H., Hadisusilo, S., Qureshi, A.A. & Elson, C.E. (1997). Isoprenoids suppress the growth of murine B16 melanomas in vitro and in vivo. Journal of Nutrition, 127, 668– 674.
Hojjati, M., & Barzegar, H. (2017). Chemical composition and biological activities of lemon (Citrus limon) leaf essential oil. Nutrition and Food Sciences Research, 4(4), 15-24.
Horvathova, E., Turcaniova, V., & Slamenova, D. (2007). Comparative study of DNA-damaging and DNA protective effects of selected components of essential plant oils in human leukemic cells K562. Neoplasma, 54(6), 478–483.
Hosseinimehr, S.J., Mahmoudzadeh, A., Ahmadi, A., Ashraf, SA., Shafaghati, N., & Hedayati, N. (2011). Te radioprotective effect of Zataria multiflora against genotoxicity induced by γ irradiation in human blood lymphocytes. Cancer Biotherapy Radiopharm, 26 (3), 325–329.
Hosseinzadeh, H., & Madanifard, M. (2000). Anticonvulsant effects of Coriandrum sativum L seeds extracts in mice. Achieves of Iranian Medicine, 3,182–184.
Kouravand, F., Jooyandeh, H., Barzegar, H., & Hojjati, M. (2018). Characterization of cross-linked whey protein isolate-based films containing Satureja Khuzistanica Jamzad essential oil. Journal of Food Processing and Preservation, 42 (3), e13557.
Lai, P.K. & Roy, J. (2004). Antimicrobial and chemo preventive properties of herbs and spices. Current Medicinal Chemistry, 11, 1451–1460.
Mahboubi, M., & Kazempour, N. (2017). Antioxidant and antimicrobial activity of Satureja khuzistanica Jamzad essential oil, ethanol and aqueous extracts. Biharean Biologist, 1-12.
Mojaddar-Langroodi A., Tajik H., & Mehdizadeh T. (2019). Antibacterial and antioxidant characteristics of Zataria multiflora Boiss essential oil and hydroalcoholic extract of Rhus coriaria L. Journal of Food Quality and Hazards Control, 6, 16-24.
Mongelli, E., Pmpuro, S., Coussio, J., Sulomon, H., & Ciccia, G. (2000). Cytotoxic and DNA interaction activities of extracts from medicinal plants used in Agrentina. Journal of Ethnopharmacology, 71,145-147.
Mohammadpour, G., Tahmasbpour, R., Noureini, S., Tahmasbpour, E., & Bagherpour, G. (2015). In vitro antimicrobial and cytotoxicity assays of Satureja bakhtiarica and Zataria multiflora essential oils. American Journal of Phytomedicine and Clinical Therapeutics, 3(6), 502-511.
Naghibi, F., Mosaddegh, M., Mohammadi Motamed, S., & Ghorbani, A. (2005). Labiatae family in folk medicine in Iran: from ethnobotany to pharmacology. Iranian Journal of Pharmaceutical Research, 2, 63-79.
Poon, S. L., McPherson, J. R., Tan, P., Teh, B. T., & Rozen, S. G. (2014). Mutation signatures of carcinogen exposure: genome-wide detection and new opportunities for cancer prevention. Journal of Genome Medicine 6:24.
Raeisi, M., Ghorbani-Bidkorpeh, F., Hashemi, M., Tepe, B., Moghaddam, Z., Aman-Mohammadi, M., & Noori, S.M.A. (2019). Chemical composition and antibacterial and antioxidant properties of essential oils of Zataria multiflora, Artemisia deracunculus and Mentha piperita. Medical Laboratory Journal, 13(2), 1-7.
Raeisi, M., Hashemi, M., Aminzare, M., Afshari, A., Zeinali, T. & Jannat, B. (2018). An investigation of the effect of Zataria multiflora Boiss and Mentha piperita essential oils to improve the chemical stability of minced meat. Veterinary world, 11, 1656-1662.
Rafieian-Kopaie, M., & Nasri, H. (2015). On the occasion of World Cancer Day 2015: the possibility of cancer prevention or treatment with antioxidants: the ongoing cancer prevention researches. International Journal of Preventive Medicine, 6, 108.
Saeedi-Saravi, S.S., & Shokrzadeh, M. (2008). The study of hepatic and renal disorders in mice which were administered ethyl acetate extract of plant Sambucus ebulus Intraperituenally (IP) and effect of vitamins C and E on prevention of its disorders. Journal of Toxicology Letters, 180, 57–58.
Saidi, M. (2014). Antioxidant Activities and Chemical Composition of Essential Oils from Satureja khuzestanica, Oliveria decumbens and Thymus daenensis. Journal of Essential Oil Bearing Plants, 17(3), 513-521.
Sefidkon, F., Naderi, M., Ghushegi, SA. (2013). Comparison of anticancer effects of nanocapsules of Nasturtium officinalis (L.) R. Br. extract with methanolic extract and its fractions. Iranian Journal of Medicinal Aromatic Plants 29(1): 35-50.
Stammati, A., Bonsi, P., Zucco, F., Moezelaar, R., Alakomi, H.L. & Von-Wright, A. (1999). Toxicity of selected plant volatiles in microbial and mammalian short-term assays. Journal of Food Chemistry and Toxicology 37: 813–823.
Sylvester, P.W. (2011). Optimization of the tetrazolium dye (MTT) colorimetric assay for cellular growth and viability. Methods of Molecular Biology, 716,157-168.
W.H.O, 2013. WHO traditional medicine strategy: (2014-2023), accses: http://www.who.int/medicines/publications/traditional/trm_strategy14_23/en.
Yang, H., & Dou, Q.P. (2010). Targeting apoptosis pathway with natural terpenoids: implications for treatment of breast and prostate cancer. Current Drug Targets, 11(6), 733-744.
Zali, H., Rezaei-Tavirani, M. & Azodi, M. (2011). Gastric cancer: prevention, risk factors and treatment. Gastroenterology Hepatology Bed Bench, 4(4), 175-185.
Zamanian-Azodi, M., Rezaie-Tavirani, M., Heydari- Kashal, S., kalantari, S., Dalilan, S. & Zali, H. (2012). Proteomics analysis of MKN45 cell line before and after treatment with Lavender aqueous extract. Gastroenterology Hepatology Bed Bench, 5(1), 35-42.
Zandi-Sohani, N., & Ramezani, L. (2015). Evaluation of five essential oils as botanical acaricides against the strawberry spider mite Tetranychus turkestani Ugarov and Nikolskii. International Biodeterioration and Biodegradation, 98, 101-106.
Zeytinoglu, M., Aydin, S., Ztrk, Y., Hsn, K. & Baser, C. (1998). Inhibitory effects of carvacrol on DMBA induced pulmonary tumorigenesis in rats. Acta Pharmaceutica Turcica, 40, 93–98.
)