Antimicrobial effects of pomegranate peel extract on Lactobacillus plantarum and shelf life of Thousand Island dressing

Document Type : Original research

Authors

1 Department of Chemical Technologies, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran

2 Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran

Abstract

Nowadays, consumers become interested to use the substitution of natural preservatives instead of chemical preservatives. It has been proven that pomegranate peel contains large amounts of antimicrobial and antioxidant compounds. In this study, the antimicrobial effect of ethanolic extract of pomegranate peel was evaluated on total microbial count, mold and yeast and Lactobacillus plantarum during 3 months storage of thousand islands dressing at 4°C. The treated sauce samples were containing different concentrations (0.05, 0.1 and 1%) of pomegranate peel extract and 0.07% sodium benzoate, potassium sorbate mixture (BS) as control. All samples were inoculated with bacterial suspension containing 105 cfu/mL of L. plantarum. MIC and MBC of pomegranate peel extract on L. plantarum were obtained 1 mg/mL. All concentrations of pomegranate peel extract significantly decreased total bacterial count (p < 0.05). The results showed that adding 0.05% pomegranate peel extract reduced 2 Log cfu/g of total mold and yeast counts compared to the control sample in souse. There was no significant difference of adding different concentration of extract on the survival of L. plantarum (p > 0.05). Adding 0.05% pomegranate peel extract did not cause undesirable sensory attributes. Pomegranate peel extract can increase the thousand islands dressing shelf life by its antimicrobial effects as a natural antimicrobial agent and preserving the probiotic properties of the product.

Keywords

Al-Zoreky, N. S. (2009). Antimicrobial activity of pomegranate (Punica granatum L.) fruit peels. International Journal of Food Microbiology, 134(3), 244–248.
Basiri, S., Shekarforoush, S. S., Aminlari, M., Abhari, K., & Berizi, E. (2014). Influence of combined vacuum packaging and pomegranate peel extract on shelf life and overall quality of pacific white shrimp (Peneous vannamei) during refrigerated storage. Iranian Journal of Veterinary Research, 15(1), 23-29.
Basiri, S., Shekarforoush, S. S., Aminlari, M., & Akbari, S. (2015). The effect of pomegranate peel extract (PPE) on the polyphenol oxidase (PPO) and quality of Pacific white shrimp (Litopenaeus vannamei) during refrigerated storage. LWT-Food Science and Technology, 60(2), 1025–1033.
Cockerill, F. R., Wikler, M. A., Alder, J., Dudley, M. N., Eliopoulos, G. M., Ferraro, M. J., Hardy, D. J., Hecht, D. W., Hindler, J. A., & Patel, J. B. (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. Clinical and Laboratory Standards Institute, Wayne, PA.
Golboie Nejad, R., Fazeli, MR, Amir Mohafari, N., Jamalifar, H. (2005). Probiotic enrichment of watermelon water using four Lactobacillus strains. In 9th Iranian Congress of Nutrition. Deputy of Food and Drug Administration (2011). Shahid Beheshti University Research and Development Department, Mayonnaise & Ketchup Technology.
Gullon, B., Pintado, M. E., Pérez-Álvarez, J. A., & Viuda-Martos, M. (2016). Assessment of polyphenolic profile and antibacterial activity of pomegranate peel (Punica granatum) flour obtained from co-product of juice extraction. Food Control, 59, 94–98.
Hosseini, S. S., Mohammadian, T., Abbaspour, M. R., Alishahi, M., & Amini, K. (2018). The effect of microencapsulation with alginate/chitosan on survival of probiotic bacteria (Lactobacillus plantarum) in the simulated condition of stomach and intestines in Huso huso. ISFJ, 27(2), 161–172.
Iranian Institute of Standards and Industrial Research (2005). Food and feed microbiology, Escherichia coli search and counting. National Iranian Standard, No. 2946.
Iranian Institute of Standards and Industrial Research (2006). Mayonnaise and salad dressings, features. National Iranian Standard, No. 2454.
Iranian Institute of Standards and Industrial Research (2007). Microbiology of food and animal feed, preparation of tests, primary suspensions and decomposition dilutions for microbiology tests, Part I. National Iranian Standards, No. 1-8923.
Jafari Khattayloo, Y., Almasi, H. (2017). Comparison of the effect of sodium benzoate and peppermint essential oil on physicochemical, microbial, sensorial and rheological properties of Mayonnaise sauce. Food Science and Technology, 15(7), 157-169.
Jooyandeh, H., Yadmellat, M. (2018). Antioxidant and antimicrobial effects of pomegranate peel extract on beef burger during chilled storage. Journal of Food Research, 28(1), 135-144.
Kanatt, S. R., Chander, R., & Sharma, A. (2010). Antioxidant and antimicrobial activity of pomegranate peel extract improves the shelf life of chicken products. International Journal of Food Science & Technology, 45(2), 216–222.
Kučerová, K., Chumchalová, J., Míková, K., Cupáková, Š., Karpíšková, R., & Ho, L. (2007). Screening of lactic acid bacteria for antimicrobial properties from mayonnaise-based products and raw materials. European Food Research and Technology, 226(1–2), 265–272.
Li, Y., Guo, C., Yang, J., Wei, J., Xu, J., & Cheng, S. (2006). Evaluation of antioxidant properties of pomegranate peel extract in comparison with pomegranate pulp extract. Food Chemistry, 96(2), 254–260.
Pederson, C. S. (1930). Bacterial spoilage of a thousand island dressing. Journal of Bacteriology, 20(2), 99.
Rahnemoon, P., Sarabi, J. M., Javanmard, D. M., & Bostan, A. (2017). Evaluation of extraction conditions on phenolic compounds and antimicrobial properties of pomegranate (Punica granatum) peels. Iranian Journal of Food Science and Technology, 14(65), 51-62.
Salahvarzi, Y., Tehranifar, A., & Jahanbakhsh, V. (2011). Relation of antioxidant and antifungal activity of different parts of pomegranate (Punica granatum L.) extracts with its phenolic content. Iranian Journal of Medicinal and Aromatic Plants, 27(1). 47-55.
Seifi, M. M., Hashemiravan, M., & Shahab, L. A. (2017). The effects of pomegranate and inulin on the survival of Bifidobacterium bifidium and the sensory and physicochemical properties of synbiotic soy sauce. Journal of Applied Microbiology in Food Industry, 3(1), 67-80.
Shahrampour, D., Khamiri, M., Kashiri, M., Razavi, M. (2017). Evaluation of antibacterial and antitumor activity of native L. plantarum isolates isolated from different nutrients. Journal of Food Science and Technology, 15, No. 85.
Shirmohammadi, M. (2014). Influence of adding barzak seed powder on some physical, chemical and sensory properties of low-fat mayonnaise sauce, Journal of Food Technology Research, 24, No. 3.
Silván, J. M., Mingo, E., Hidalgo, M., de Pascual-Teresa, S., Carrascosa, A. V, & Martinez-Rodriguez, A. J. (2013). Antibacterial activity of a grape seed extract and its fractions against Campylobacter spp. Food Control, 29(1), 25–31.
Singh, B., Singh, J. P., Kaur, A., & Singh, N. (2018). Phenolic compounds as beneficial phytochemicals in pomegranate (Punica granatum L.) peel: A review. Food Chemistry, 261, 75–86.
Smittle, R. B. (2000). Microbiological safety of mayonnaise, salad dressings, and sauces produced in the United States: a review. Journal of Food Protection, 63(8), 1144–1153.
Sorrenti, V., Randazzo, C. L., Caggia, C., Ballistreri, G., Romeo, F. V., Fabroni, S., Timpanaro, N., Raffaele, M., & Vanella, L. (2019). Beneficial effects of pomegranate peel extract and probiotics on pre-adipocyte differentiation. Frontiers in Microbiology, 10.
Vaithiyanathan, S., Naveena, B. M., Muthukumar, M., Girish, P. S., & Kondaiah, N. (2011). Effect of dipping in pomegranate (Punica granatum) fruit juice phenolic solution on the shelf life of chicken meat under refrigerated storage (4 C). Meat Science, 88(3), 409–414.
Wijngaard, H., Hossain, M. B., Rai, D. K., & Brunton, N. (2012). Techniques to extract bioactive compounds from food by-products of plant origin. Food Research International, 46(2), 505–513.