TaqMan real-time PCR: a reliable method to detect meat species

Document Type: Original research

Authors

1 Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

2 Central Laboratory of Veterinary Head Office of Khorasan Razavi Province, Iran

3 Central Laboratory of Veterinary Head Office of North Khorasan Province, Iran

Abstract

The aim of this study was to detect and identify meat species (beef, lamb, pork, chicken, donkey, and horse) in Kebab as a Halal meat-based food sold in on-road-restaurants of North Khorasan province (Iran) using TaqMan real-time polymerase chain reaction (PCR). Raw Kebab samples (150 samples) were obtained from on-road restaurants. The samples were prepared, DNA was extracted and TaqMan real-time PCR using beef, lamb, pork, chicken, horse, and donkey target genes was performed. The results indicated that 100% of samples yielded positive results of beef, but none of them generated donkey and pork amplicons. The samples of Kebab indicated 93.33% lamb, 83.33% chicken, 10% horse positive results. The information claimed for the source of meat was not correct for more than 95% of the samples and only one food center labeled the Kebab mix correctly. Except for one Kebab sample that showed a positive result for the presence of horse meat, no sample contained meat of species considered illegal in Halal foods.

Keywords


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