Effect of edible coatings based on zein and chitosan and the use of Roman aniseed oil on the microbial activity of Mazafati dates

Document Type: Original research


1 Agricultural Engineering Research Department, Kerman Agricultural and Resource Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran

2 Agricultural Engineering Research Institute (AERI), Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

3 Seed and Plant Improvement Research Department, Kerman Agricultural and Natural Resources Research and Education Centre, Agricultural Research, Education and Extension Organization (AREEO), Kerman, Iran



Maintaining optimum quality and eliminating dates of contamination, cleanup and packaging to extend the shelf life is one of the country's priorities and policies. Edible coatings with vegetable oils are one way to prevent the growth of microorganisms. In this study, the influence of the coating with natural zein and chitosan polymers along with the essential oil of Roman anise on the microbial behavior of Matsafati dates during one-year storage at 2 and 4 °C and a second year at 10 °C was examined. The results showed that the microbial sample of Bam region with 32% moisture content had a high microbial burden. Aspergillus niger, Aspergillus flavus, Rhizopus stolonifer, and Alternaria alternata were growing on it. These fungi, especially Aspergillus niger were involved in the rotting and lactic acid bacteria were involved in the rancidity of Mazafat dates. In this study, the number of Aspergillus niger fungi using edible coatings of zein and chitosan as well as anise oil in three logarithmic cycles was reduced and the Penicillium fungi were eliminated. In general, the lowest microbial growth was observed in zein treatment and the highest in the control sample. The use of anise oil had the effect of reducing the total count of microorganisms. According to microbial results, the use of zein treatment is recommended.


Adams, M. R., & Moss, M. O. (2000). Food Microbiology. The Royal Society of Chemistry, U. K. pp. 479.
Afshari Jouybari, H., & Farahnaky, A. (2011). Accelerated ripening of Mazafati date by hot water, acetic acid and sodium chloride solutions. Journal of Food Science & Technology, 8(30), 45-52.
Ahmadi, K., Ebadzadeh, H. R., Hatami, F., Hoseinpour, R., & Abdeshah, H. (2018). Agricultural statistics: horticultural products. Ministry of Agricultural Jihad, Plannining and Economic Affairs, Information Technology and Communication Center, pp. 129.
Ahmed, I., Ahmed, A., & Robinson, R. K. (1997). Susceptibility of date fruits (Phoenix dactylifera) to aflatoxin production. Journal of the Science of Food and Agriculture, 74, 64-68.
Al- Shaickly, M. A. S., Al- Rubaie, I. A., & Al- Dulaimi, A. A. (1986).Types and extent of microbial contamination on fresh Iraqi dates during maturation. Date Palm Journal, 4(2), 205-220.
Bolin, H. R., King, A. D., Stanley, W. L., & Jurd, L. (1972). Antimicrobial protection of moisturized Deglet Noor dates. Applied Microbiology, 23(4), 799-802.
Davidson, P. M., & Zivanovic, S. (2003). The use of natural antimicrobials. Pp. 5-8. In: Zeuthen P and Bogh Sorensen L. Food preservation techniques. Woodhead Publishing Limited and CRC Press, Washington.
Delkhah, H., Rezaee, H., Borhan, M., & Kohan, G. R. (2012). Alternatives to methyl bromide in stored dates. 1st Conference on Date Palm and Food Security, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran, 17-18 Oct. 2012.
Ghanbarzadeh, B., Oromiehie, A. R., Musavi, M., Falcone, P. M., Emam Djomeh, Z., & Rad, E. R. (2007). Study of mechanical properties, oxygen permeability and AFM topography of zein films plasticized by polyols. Packaging Technology and Science, 20, 155-163.
Golshan Tafti, A., & Fouladi, M. H., (2006). Preservation of Mazafati Rutab using authorized antimicrobial agents. Iranian Journal of Agriculture Science, 37(1), 131-137.
Golshan Tafti, A., Damankeshan, B., & Shafee, L. (2015). Botany and properties of Mazafati date fruit. Ministry of Agricultural Jihad, Kerman Agricultural Jihad Organization.
Golshan Tafti, A., Solaimani Dahdivan, N., & Azizi, A. (2019). The effect of acetic acid and sodium diacetate solutions on artificial ripening of Mazafati date fruit. Journal of Food Science and Technology, 85(15), 349-356.
Gross, M., Friedman, J., Dudai, N., Larkov, O., Cohen, Y., & Bar, E. (2002). Biosynthesis of estragole and t-anethole in bitter fennel (Foeniculum vulgare Mill. var. vulgare) chemotypes. Changes in SAM: phenylpropene omethyltranferase activities during development. Plant Science, 163, 1047-1053.
Han, C., Zhao, Y., Leonard, S. W., & Traber, M. G. (2004). Edible coating to improve storability and enhance nutritional value of fresh and frozen strawberries (Fragaria xananassa) and raspberries (Rubus ideaus). Postharvest Biology and Technology, 33, 67-78.
Ildiko, T. (2006). Alternative polymers and processes for coating. Ph.D dissertation. Der Freien Universitat, Berlin.
Jalili, H. (2005). Determination of optimum method for preparation, packaging and storage of dates in Fars province. Agricultural Engineering Research Institute, No. 1384/1392, 19 pages.
Janes, M. E., Kooshesh, S., & Johnon, M. G. (2002). Control of Listeria monocytogenes on the surface of refrigerated, ready- to- eat chicken coated with edible zein film coatings containing nisin and/or calcium propionate. Journal of Food Science, 67, 2754-2757.
Joerger, R. D. (2007). Antimicrobial films for food application: A quantitative analysis of their effectiveness. Packaging Technology and Science, 20, 231-273.
Karim, G. 2015. Food microbial tests. 6th Edition, Tehran University Publication. 536 pages. (In Persian)
Khodabakhshian Kargar, R., & Emadi, B. (2014). Evaluation of some physical and chemical properties of Mozafati date fruit at different stages of maturity. The 8th National Congress on Agricultural Machinery Engineering (Biosystem) & Mechanization, Mashhad Ferdousi University, 2014/01/29.
Kim, S. H., No, H. K., Kim, S. D., & Prinyawiwatkul, W. (2006). Effect of plasticizer concentration and solvent types on shelf- life of eggs coated with chitosan. Journal of Food Science, 71, S249-353.
Majeti, N. V., & Kumar, R. (2000). A review of chitin and chitosan applications. Reactive and Functional Polymers, 46, 1-27.
No, H. K., Meyers, S. P., Prinyawiwatkul, W., & Xu, Z. (2007). Applications of chitosan for improvement of quality and shelf of foods: A review. Journal of Food Science, 72, 87-100.
Ryu, K. N., Kim, S. H., No, H. K., & Prinyawiwakul, W. (2009). Effects of storage temperature on quality of eggs coated with chitosan. Journal of Chitin and Chitosan, 14, 143-148.
Salajegheh, F. (2013). Evaluating of microbial quality of Mozafati Rutab upon storage. Agricultural Engineering Research Institute, Final Research Report, No. 42171.
Shahidi, F., & Abuzaytoun, R. (2005). Chitin, chitosan, and co- products: chemistry, production, application, and health effects. Advances in Food and Nutrition Research, 49, 93-135.
Shenasi, M., Aidoo, K. E., & Candlish, A. A. (2002). Microflora of date fruits and production of aflatoxins at various stages of maturation. International Journal of Food Microbiology, 79(1-2), 113-119.
 Tajik, H., Moradi, M., Razavi Rohani, S. M., Erfani, A. M., & Sabet Jalali, F. S. (2008). Preparation of chitosan from brine shrimp (Artemia urmiana) cyst shells and effects of different chemical processing sequences on the physicochemical and functional properties of the product. Molecules, 13, 1263-1274.
Vahedikia, N., Garavand, F., Tajeddin, B., Cacciotti, I., Jafari, S. M., Omidi, T., & Zahedi, Z. (2019). Biodegradable zein film composites reinforced with chitosan nanoparticles and cinnamon essential oil: physical, mechanical, structural and antimicrobial attributes. Colloids and Surfaces B: Biointerface, 177, 25-32.
Xie, L., Hettiarachchy, N. S., Ju, Z. Y., Meullenet, J., Wang, H., Slabvik, M. F., & Janes, M. E. (2002). Edible film coating to minimize eggshell breakage and reduce post- wash bacterial contamination measured by dye penetration in eggs. Journal of Food Science, 67, 280-284.