Journal of Food and Bioprocess Engineering

Comparison of active ingredients properties in callus culture of Zataria multiflora with native plant

Document Type : Original research

Authors

1 Department of Chemical Engineering, Jundi-Shapur University of Technology, Dezful, Iran

2 Iran Dairy Industries (Pegah), Khuzestan, Iran

3 Department of Agronomy & Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan, Iran

Abstract

Recent studies show that the extraction of active ingredients from medicinal plants is subject to various parameters includes plant type, cultivation conditions and the extraction method. This research aimed to compare the callus cultivated of Zataria multiflora with its native plant. Thus, Murashige and Skoog (MS) medium were used to grow the plant in vitro and then the callus was obtained. Then a combined ultrasound-microwave method was applied to extract the chemical compounds of the native plant as well as its callus. Herein, extraction conditions were 20 min for ultrasound time, 350 watts for ultrasonic power, and 800 watts for microwave power. According to the results, the cultivated callus obtained from Zataria multiflora could not produce measurable amounts of oily compounds found in the native plant. Therefore, callus extracts were studied in terms of antioxidant and antimicrobial activity. The results of gas chromatography-mass spectrometry analysis showed that the highest compositions for essential oil of the native plant were carvacrol (86.1%) and thymol (2.01%). Whereas, the major chemical compounds for callus extract were also carvacrol (71.65%) and thymol (11.25%). In addition, the study of antioxidant activity by DPPH method revealed that the callus has a weaker antioxidant activity than the native plant. Whereas, the results were opposite in the case of antimicrobial characteristics. To put it another way, callus did better in antimicrobial properties.

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Main Subjects

Anwar, M., Birch, E. J., Ding, Y., & Bekhit, A. E. D. (2022). Water-soluble non-starch polysaccharides of root and tuber crops: Extraction, characteristics, properties, bioactivities, and applications. Critical reviews in food science and nutrition, 62(9), 2309-2341.
Babich, O., Sukhikh, S., Pungin, A., Ivanova, S., Asyakina, L., & Prosekov, A. (2020). Modern trends in the in vitro production and use of callus, suspension cells and root cultures of medicinal plants. Molecules, 25(24), 5805.
Chiocchio, I., Mandrone, M., Tomasi, P., Marincich, L., & Poli, F. (2021). Plant secondary metabolites: An opportunity for circular economy. Molecules, 26(2), 495.
Cockerill, F. R., Wikler, M. A., Alder, J., Dudley, M. N., Eliopoulos, G. M., Ferraro, M. J., ... & Zimmer, B. L. (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. Clinical & Laboratory Standards Institute, 32, M07-A9.
Dezaki, E. S., Mahmoudvand, H., Sharififar, F., Fallahi, S., Monzote, L., & Ezatkhah, F. (2016). Chemical composition along with anti-leishmanial and cytotoxic activity of Zataria multiflora. Pharmaceutical Biology, 54(5), 752–758.
Ebrahimzadeh, H., Yamini, Y., Sefidkon, F., Chaloosi, M., & Pourmortazavi, S. M. (2003). Chemical composition of the essential oil and supercritical CO2 extracts of Zataria multiflora Boiss. Food Chemistry, 83(3), 357–361.
Espín, J. C., Soler-Rivas, C., & Wichers, H. J. (2000). Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. Journal of Agricultural and Food Chemistry, 48(3), 648–656.
Fathi Rezaei, P., & Rakee, E. (2018). Investigation of sucrose effect on tropane alkaloid production and several biochemical parameters of Datura under in vitro culture condition. Molecular and Cellular Researches, 30(4), 375–386.
Gavahian, M., Farahnaky, A., Majzoobi, M., Javidnia, K., Saharkhiz, M. J., & Mesbahi, G. (2011). Ohmic-assisted hydrodistillation of essential oils from Zataria multiflora Boiss (Shirazi thyme). International Journal of Food Science and Technology, 46(12), 2619–2627.
Golmakani, M. T., & Rezaei, K. (2008). Microwave-assisted hydrodistillation of essential oil from Zataria multiflora Boiss. European Journal of Lipid Science and Technology, 110(5), 448–454. https://doi.org/10.1002/ejlt.200700239
Güllüce, M., Sökmen, M., Daferera, D., Aǧar, G., Özkan, H., Kartal, N., Polissiou, M., Sökmen, A., & Şahin, F. (2003). In vitro antibacterial, antifungal, and antioxidant activities of the essential oil and methanol extracts of herbal parts and callus cultures of Satureja hortensis L. Journal of Agricultural and Food Chemistry, 51(14), 3958–3965.
Karimi, S., Sharifzadeh, S., & Abbasi, H. (2020). Sequential ultrasound-microwave assisted extraction as a green method to extract essential oil from Zataria multiflor. Journal of Food Bioprocess Engineering, 3(2), 101–109.
Khalili, G., Mazloomifar, A., Larijani, K., Tehrani, M. S., & Azar, P. A. (2018). Solvent-free microwave extraction of essential oils from Thymus vulgaris L. and Melissa officinalis L. Industrial Crops and Products, 119, 214–217.
Khosravinia, S., Bagheri, A., & Ziaratnia, S. M. (2015). Investigation of antimicrobial activity of extracts obtained from callus and cell suspension culture of Bunium persicum compared to seed and effect of solvent and dilution of the extract on the activity. Crop Biotechnology, 5(9), 39-48.
Koufan, M., Belkoura, I., Mazri, M. A., Amarraque, A., Essatte, A., Elhorri, H., Zaddoug, F., & Alaoui, T. (2020). Determination of antioxidant activity, total phenolics and fatty acids in essential oils and other extracts from callus culture, seeds and leaves of Argania spinosa (L.) Skeels. Plant Cell, Tissue and Organ Culture, 141(1), 217–227.
Makkizadeh Tafti, M., Naghdi Badi, H., Rezazadeh, S., Ajani, Y., & Kadkhoda, Z. (2010). Evaluation of botanical traits and oil content/chemical composition in Iranian Thymus carmanicus jalas ecotypes. Journal of Medicinal Plants, 9(36), 57–65.
Maslova, E., Glodik, T., Semykina, V., Gulya, N., & Perelygina, T. (2019, November). Study of antimicrobial activity of the callus tissue Salvia pratensis L.(Lamiaceae) in vitro. In 1st International Symposium Innovations in Life Sciences (ISILS 2019) (pp. 303-306). Atlantis Press.
Memar, M. Y., Raei, P., Alizadeh, N., Aghdam, M. A., & Kafil, H. S. (2017). Carvacrol and thymol: strong antimicrobial agents against resistant isolates. Reviews in Medical Microbiology, 28(2), 63-68.
Morales, J. M. (2002). Behavior at habitat boundaries can produce leptokurtic movement distributions. American Naturalist, 160(4), 531–538.
Mosafa, N. (2016). The study of antioxidant properties of Lavandula angustifolia in tissue culture. The Journal of Plant Research, 28(4), 835–843.
Nakhaee Moghadam, M., Movaffagh, J., Fazly Bazzaz, B. S., Azizzadeh, M., & Jamshidi, A. (2020). Encapsulation of Zataria multiflora essential oil in saccharomyces cerevisiae: Sensory evaluation and antibacterial activity in commercial soup. Iranian Journal of Chemistry and Chemical Engineering, 39(2), 233–242.
Nazir, S., Jan, H., Tungmunnithum, D., Drouet, S., Zia, M., Hano, C., & Abbasi, B. H. (2020). Callus culture of Thai basil is an effective biological system for the production of antioxidants. Molecules, 25(20), 4859.
Picot-Allain, C., Mahomoodally, M. F., Ak, G., & Zengin, G. (2021). Conventional versus green extraction techniques—A comparative perspective. Current Opinion in Food Science, 40, 144-156.
Shams Ardakani M.R., & Pourshafiei, F. (2003). Cell culture production of Nepeta persica boiss and comparison between its secondary metabolites and intact plant. Journal of Research In Medical Sciences (JRMS), 7(2), 147–149.
Shams Ardekani, M. R., Hadjiakhoondi, A., Jamshidi, A. H., & Abdi, K. H. (2005). The study of volatile oil of Foeniculum vulgare Miller. In their tissue culture and comparison with the whole plant. Journal of Medicinal Plants, 4(15), 73-80.
Sharifzadeh, S., Karimi, S., Abbasi, H., & Assari, M. (2022). Sequential ultrasound-microwave technique as an efficient method for extraction of essential oil from Lavandula coronopifolia Poir. Journal of Food Measurement and Characterization, 16(1), 377-390.
Patil, S. M., Kumari, V. C., Sumana, K., Sujay, S., Tejaswini, M., Shirahatti, P. S., & Ramu, R. (2021). Sustainable development of plant tissue culture industry: The Indian scenario. Journal of Applied Biology and Biotechnology, 9(2), 1-7.
Sökmen, M., Serkedjieva, J., Daferera, D., Gulluce, M., Polissiou, M., Tepe, B., Akpulat, H. A., Sahin, F., & Sokmen, A. (2004). In vitro antioxidant, antimicrobial, and antiviral activities of the essential oil and various extracts from herbal parts and callus cultures of Origanum acutidens. Journal of Agricultural and Food Chemistry, 52(11), 3309–3312.
Thormar, H., & Hilmarsson, H. (2011). Antimicrobial lipids as disinfectants, antiseptics and sanitizers. Lipids and Essential Oils as Antimicrobial Agents, 179-201.
Wyk, B.-E., & Wink, M. (2017). Medicinal Plants of the World - Ben-Erik van Wyk, Michael Wink - Google Books. Cabi International, 322–323.
Zaman, M. A. K., Azzeme, A. M., Ramle, I. K., Normanshah, N., Ramli, S. N., Shaharuddin, N. A., Ahmad, S., & Abdullah, S. N. A. (2020). Induction, multiplication, and evaluation of antioxidant activity of polyalthia bullata callus, a woody medicinal plant. Plants, 9(12), 1–21.
Journal of Food and Bioprocess Engineering
Volume 5, Issue 1
June 2022
Pages 86-92