Enzymatic Dehulling of Sesame (Sesamum indicum L.) Seeds Using Cellulase and Xylanase
Document Type : Original research
Authors
1 Chemical and polymer engineering Yazd university,Yazd,Iran
2 1- Department of Chemical Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman, Iran
Abstract
Enzymatic dehulling of sesame (Sesamum indicum L.) seeds was investigated in this research work. Since cellulose and hemicellulose are two main ingredients of sesame hull, cellulase and xylanase were used for sesame seeds dehulling. A full factorial experimental design was employed to investigate the effects of total enzyme activity in solution, the ratio of cellulase to xylanase activity, and mass percentage of seeds in solution on the dehulling process. Analysis of variance showed that all the three factors affected the process significantly. Efficiencies as high as 95% dehulling in 15 min was obtained with total enzyme activity of 10 U/ (g seeds), the cellulase to xylanase activity ratio of 1, and the sesame seed mass percentage in solution of 10% (w/w). In a separate experiment the rate of dehulling was determined as a function of time and seeds mass percentage in solution, and a rate equation was suggested for the rate of dehulling. Investigations, moreover, showed that the spent enzyme solution from one batch of dehulling could be reused up to four times without significant loss of enzyme activity.
Keywords
Main Subjects
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