Validation of an Analytical Method to Determine the Content of Ochratoxin A in malt extract and malt beverage
Document Type : Original research
Authors
1 Department of Food Toxicology, Research Center of Food Technology and Agricultural Products, Standard Research Institute, Karaj, Iran
2 Department of Food Science and Technology Standard Research Institute (SRI), Karaj, Iran, z.alaei@standrd.ac.ir
3 Standard Research Institute, Research Center of Food Technology and Agricultural Products, Department of Food Toxicology
Abstract
This study aims to develop a method for the determination of ochratoxin A in malt extract and malt beverage samples based on the extraction of the samples with acetonitrile solution, followed by immunoaffinity cleanup and determination by high-performance liquid chromatography with fluorescence detection at an excitation of 333 nm and emission of 477 nm. The proposed method was validated to assess the linearity, limit of detection, limit of quantitation, precision, and recovery. The results demonstrated that the procedure was suitable for determining ochratoxin A in these samples and could be performed for their routine analysis in mycotoxin laboratories. The study involved 6 participants representing a cross-section of research, private, and official control laboratories from accredited laboratories in Iran. The method showed acceptable results within-laboratory and between-laboratory precision for each matrix, as required by the codex manual.
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